Mulhouse ( mə-LOOZ, French: [myluz] ; Alsatian: Mìlhüsa [mɪlˈhyːsa]; German: Mülhausen [myːlˈhaʊzn̩] , meaning "mill house") is a French city of the European Collectivity of Alsace (Haut-Rhin department, in the Grand Est region of France). It is near the border with Switzerland and Germany. It is the largest city in Haut-Rhin and second largest in Alsace after Strasbourg.

Mulhouse is known for its museums, especially the Cité de l'Automobile (also known as the Musée national de l'automobile, 'National Museum of the Automobile') and the Cité du Train (also known as Musée Français du Chemin de Fer, 'French Museum of the Railway'), respectively the largest automobile and railway museums in the world. An industrial town nicknamed "the French Manchester", Mulhouse is also the main seat of the Upper Alsace University, where the secretariat of the European Physical Society is found.

Mulhouse is a commune with a population of 108,312 in 2019.